Frequently Asked Questions
- Recommendations from Parent's Guide to Cord Blood
- Donate Cord Blood
- Find a Family Bank
- Collecting Cord Blood
- Shipping & Handling
- Processing Methods
- Laboratory Standards
- Storage Facility
- Consumer Protection
- Questions Parents should ask a Family Bank
- Stem Cell Transplants with Cord Blood
- Regenerative Medicine with Cord Blood
- See all FAQs
Collecting Cord Blood
There are two methods of collection in common use. One is to hang a blood bag lower than the mother and let gravity draw blood down the tube into the bag. This method is used in most countries of the world, because it has the fewest steps, and therefore the fewest opportunities for mistakes or contamination.
The second method is to actively draw the blood out, just like when a person has a blood draw for a medical test. The draw can be done with a standard syringe or with a bulb in the bag tubing that creates suction. Studies have shown that actively drawing the blood will collect a larger volume faster.
Sun, JJ et al., Transfusion Sept. 2010; 50(9):1980-1987
- What instructional tools are provided for the physician and delivery staff?
- Will the cord blood company actively contact the labor and delivery staff for you -- or are parents responsible for keeping them informed and coordinated?
- What collection method do they use: gravity drip or blood draw?
- Is the collection blood bag sterile, both inside and out, so that it can be used in the operating room for a C-section?
- Do they provide the option of collecting additional stem cells from the placenta or tissue of the umbilical cord?
2. Sterilize before every needle stick. When in doubt, sterilize again!
3. Volume, volume, volume. You want to "milk the cord" for as much blood as possible. If the blood vessel you are using stops working, try another or move upstream, but sterilize first.
1.3 mL of cord blood for every pound of patient weight, -or-
2.9 mL of cord blood for every kg of patient weight
However, as more transplant centers are adopting the practice of giving adult patients "double cord blood transplants" with two cord blood units, it is less critical for both units to have adequate cell dose.
Reed, W et al., Blood 2003;101(1):351
Barker, JN et al., Blood 2005;105:1343-1347
Eapen, M et al. Lancet 2007;369:1947-54
Rocha & Gluckman Brit. J. Haematology 2008;147:262-274
Delaney, C et al., Brit. J. Haematology 2009;147:207-216
Michallet et al. 2010 Blood 2010;116:Abstract#361
Hutton, EK & Hassan, ES, JAMA 2007; 297:1241-1252
van Rheenen, P et al., Tropical Med. and Internal Health 2007; 12(5):603-616
Abalos E., 2009; The World Health Organization Reproductive Health Library